View bacteriocin : Halocin-S8

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Accession BAC055
Name Halocin-S8
Related Entries Halocin-C8Halocin H4
Gene halS8
Class Unclassified
Producer Organism Haloarchaeon S8a [Gram-]
Taxonomy ArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceae
Target organisms haloarchaeons Halobacterium salinarium NRC817 - Halobacterium GRB - Haloferax gibbonsii
Swiss-Prot Entry Q9HHA8
PDB Entry Unknown
Other databases EMBL AF276080InterPro IPR006311TIGRFAMs TIGR01409
Description Mode of action:
boiled, subjected to organic solvents, and stored at 4 degrees Celsius for extended periods without losing activity.
[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 231-266.
MEDLINE=20398181;PubMed=10940040;DOI=10.1128/JB.182.17.4951-4958.2000
Price L.B., Shand R.F.
"Halocin S8: a 36-amino-acid microhalocin from the haloarchaeal strain S8a.", J. Bacteriol. 182:4951-4958(2000).

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Sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 SDCNINSNTA ADVILCFNQV GSCALCSPTL VGGPVP
Wheel representation
Composition
Formula C150 H243 N41 O52 S4
Absent amino acids EHKMRWY
Common amino acids CNSV
Mass (Da) 3599.68
Net charge -2
Isoelectric point 3.49
Basic residues 0
Acidic residues 2
Hydrophobic residues 13
Polar residues 17
Aliphatic residues 9
Tiny residues 10
Boman Index -11.17
Hydropathy Index 0.56
Aliphatic Index 94.72
Instability Index 49.04 (unstable)
Half Life Mammalian : 1.9 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 250 M-1 cm-1
Absorbance 280nm 7.14
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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