View bacteriocin : Microcin B17 (MccB17)

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Accession BAC072
Name Microcin B17 (MccB17)
Related Entries Microcin J25Microcin H47Microcin E492Colicin-V (Microcin-V)Microcin-24

Microcin C7
Gene mcbA
Class Unclassified
Producer Organism Escherichia coli [Gram-negative]
Taxonomy BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Target organisms Unavailable data
Swiss-Prot Entry P05834 Q7M024
PDB Entry 2MLP resolved by NMR
Other databases EMBL M15469EMBL M24253EMBL X06417PIR A25219PIR A58375
Description Mode of action:
This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.

post-translational modification:
The processed N-terminus does not resemble a typical secretion signal sequence.

The Cys residues as well as some Gly and Ser are post- translationally modified. Modifications include the formation of four thiazole and four oxazole rings that result, respectively, from the condensation of four cysteine and four serine side chains with the carbonyl group of the preceding amino acid.
[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
MEDLINE=88217867;PubMed=3329729;DOI=10.1002/prot.340010305
Davagnino J., Herrero M., Furlong D., Moreno F., Kolter R.
"The DNA replication inhibitor microcin B17 is a forty-three-amino- acid protein containing sixty percent glycine.", Proteins 1:230-238(1986).

[Ref. 2] | View abstract | Export citation
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=89123111;PubMed=2644225;
Genilloud O., Moreno F., Kolter R.
"DNA sequence, products, and transcriptional pattern of the genes involved in production of the DNA replication inhibitor microcin B17.", J. Bacteriol. 171:1126-1135(1989).

[Ref. 3] | View abstract | Export citation
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
MEDLINE=88216163;PubMed=2835580;
Conell N., Han Z., Moreno F., Kolter R.
"An E. coli promoter induced by the cessation of growth.", Mol. Microbiol. 1:195-201(1987).

[Ref. 4]
PARTIAL PROTEIN SEQUENCE OF 27-69.
Bayer A., Stevanovic S., Freund S., Metzger J.W., Jung G.
"Isolation and structure elucidation of the 43-peptide antibiotic microcin B17.",

[Ref. 5] | View abstract | Export citation
PROTEIN SEQUENCE OF 27-38, AND POST-TRANSLATONAL MODIFICATIONS.
MEDLINE=94240167;PubMed=8183941;
Yorgey P., Lee J., Koerdel J., Vivas E., Warner P., Jebaratnam D., Kolter R.
"Posttranslational modifications in microcin B17 define an additional class of DNA gyrase inhibitor.", Proc. Natl. Acad. Sci. U.S.A. 91:4519-4523(1994).

[Ref. 6] | View abstract | Export citation
FUNCTION.
MEDLINE=91122055;PubMed=1846808;
Vizan J.L., Hernandez-Chico C., del Castillo I., Moreno F.
"The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.", EMBO J. 10:467-476(1991).

[Ref. 7] | View abstract | Export citation
STRUCTURE BY NMR OF 1-26.
MEDLINE=98213789;PubMed=9545435;DOI=10.1016/S1074-5521(98)90635-4
Roy R.S., Kim S., Baleja J.D., Walsh C.T.
"Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.", Chem. Biol. 5:217-228(1998).

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Sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 VGIGGGGGGG GGGSCGGQGG GCGGCSNGCS GGNGGSGGSG SHI
Wheel representation
Structure
Composition
Formula C118 H188 N48 O53 S4
Absent amino acids ADEFKLMPRTWY
Common amino acids G
Mass (Da) 3274.06
Net charge +1
Isoelectric point 7.12
Basic residues 1
Acidic residues 0
Hydrophobic residues 3
Polar residues 38
Aliphatic residues 3
Tiny residues 32
Boman Index -0.44
Hydropathy Index -0.13
Aliphatic Index 24.88
Instability Index 60.57 (unstable)
Half Life Mammalian : 100 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 250 M-1 cm-1
Absorbance 280nm 5.95
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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