View bacteriocin : Microcin E492

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Accession BAC119
Name Microcin E492
Related Entries Microcin J25microcin B17 (MccB17)Microcin H47Colicin-V (Microcin-V)Microcin-24

Microcin C7
Gene mceA
Class Unclassified
Producer Organism Klebsiella pneumoniae [Gram-negative]
Taxonomy BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
Target organisms enterobacteria
Swiss-Prot Entry Q9Z4N4 P82962
PDB Entry Unknown
Other databases EMBL AF063590
Description Mode of action:
Channel-forming bacteriocin. Forms cation-selective channels.

Post-translational modification:
The C-terminal Ser is modified by attachment to a siderophore similar to enterobactin, which can bind one atom of iron. The modification consists of an ester linkage of the serine carboxyl to O6 of a glucose which is linked by a C-glycosidic bond to the 5'-benzoyl of a linear triester of N-(2,3-dihydroxybenzoyl)serine. RANGE=20-103; NOTE=Ref.2. 103 unmodified form (Ref.3). 103 modified form (Ref.3).
[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION, STRAIN=RYC492
MEDLINE=99084959;PubMed=9864332;
Lagos R., Villanueva J.E., Monasterio O.
"Identification and properties of the genes encoding microcin E492 and its immunity protein.", J. Bacteriol. 181:212-217(1999).

[Ref. 2]
PROTEIN SEQUENCE OF 20-103, AND MASS SPECTROMETRY.
Pons A.M., Vignon D., Cottenceau G.
"", Submitted (FEB-2001) to Swiss-Prot.

[Ref. 3] | View abstract | Export citation
PARTIAL PROTEIN SEQUENCE, AMINO-ACID COMPOSITION, SIDEROPHORE BINDING SITE, AND MASS SPECTROMETRY, STRAIN=RYC492
PubMed=15102848;DOI=10.1074/jbc.M400228200
Thomas X., Destoumieux-Garzon D., Peduzzi J., Afonso C., Blond A., Birlirakis N., Goulard C., Dubost L., Thai R., Tabet J.-C., Rebuffat S.
"Siderophore peptide, a new type of post-translationally modified antibacterial peptide with potent activity.", J. Biol. Chem. 279:28233-28242(2004).

[Ref. 4] | View abstract | Export citation
CHARACTERIZATION, STRAIN=RYC492
MEDLINE=93238942;PubMed=7682973;DOI=10.1016/0014-5793(93)80096-D
Lagos R., Wilkens M., Vergara C., Cecchi X., Monasterio O.
"Microcin E492 forms ion channels in phospholipid bilayer membrane.", FEBS Lett. 321:145-148(1993).

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Sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 GETDPNTQLL NDLGNNMAWG AALGAPGGLG SAALGAAGGA LQTVGQGLID HGPVNVPIPV LIGPSWNGSG SGYNSATSSS
 GSGS
Wheel representation
Composition
Formula C338 H530 N98 O118 S1
Absent amino acids CFKR
Common amino acids G
Mass (Da) 7906.02
Net charge -3
Isoelectric point 3.7
Basic residues 1
Acidic residues 4
Hydrophobic residues 28
Polar residues 41
Aliphatic residues 16
Tiny residues 39
Boman Index -26.98
Hydropathy Index 0.01
Aliphatic Index 81.43
Instability Index 21.21 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 12490 M-1 cm-1
Absorbance 280nm 150.48
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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