View bacteriocin : Colicin-M

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Accession BAC135
Name Colicin-M
Related Entries Colicin-V (Microcin-V)Colicin-E1Colicin-10Colicin-NColicin-Ia

Colicin-Ib
Gene cma
Class Unclassified
Producer Organism Escherichia coli [Gram-negative]
Taxonomy BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Target organisms Enterobacteriaceae - Escherichia coli
Swiss-Prot Entry P05820
PDB Entry 2XMX resolved by X-RAY 2XTQ resolved by X-RAY 2XTR resolved by X-RAY 3DA3 resolved by X-RAY 3DA4 resolved by X-RAY
Other databases EMBL M16754PIR A27090InterPro IPR010916InterPro IPR010917PROSITE PS00430

PROSITE PS01156
Description colicin M contains a pentapeptide (Glu-Thr-Leu-Thr-Val) in the domain required for uptake into cells.
A similar sequence was found in all colicins which are taken up by a TonB-dependent mechanism and in outer membrane receptor proteins which are constituents of TonB-dependent transport systems.
The structure of colicin M in the carboxy-terminal activity domain had no resemblance to the pore-forming colicins or colicins with endonuclease activity.
[Ref. 1] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, [GENOMIC DNA].
MEDLINE=87250310;PubMed=3036784;
RA Koeck J., Oelschlaeger T., Kamp R.M., Braun V.
"Primary structure of colicin M, an inhibitor of murein biosynthesis.", J. Bacteriol. 169:3358-3361(1987).

[Ref. 2] | View abstract | Export citation
3D STRUCTURE
PubMed=21149455;DOI=10.1074/jbc.M110.165274
Helbig S., Patzer S.I., Schiene-Fischer C., Zeth K., Braun V.
"Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone.", J.Biol.Chem. 286: 6280-6290 (2011).

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Sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 METLTVHAPS PSTNLPSYGN GAFSLSAPHV PGAGPLLVQV VYSFFQSPNM CLQALTQLED YIKKHGASNP LTLQIISTNI
 GYFCNADRNL VLHPGISVYD AYHFAKPAPS QYDYRSMNMK QMSGNVTTPI VALAHYLWGN GAERSVNIAN IGLKISPMKI
 NQIKDIIKSG VVGTFPVSTK FTHATGDYNV ITGAYLGNIT LKTEGTLTIS ANGSWTYNGV VRSYDDKYDF NASTHRGIIG
 ESLTRLGAMF SGKEY
Structure
Composition
Formula C1324 H2059 N353 O392 S9
Absent amino acids
Common amino acids GSLIT
Mass (Da) 29505.15
Net charge +13
Isoelectric point 9.5
Basic residues 30
Acidic residues 17
Hydrophobic residues 89
Polar residues 104
Aliphatic residues 54
Tiny residues 65
Boman Index -276.94
Hydropathy Index -0.13
Aliphatic Index 86.01
Instability Index 38.09 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 33475 M-1 cm-1
Absorbance 280nm 123.98
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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