View bacteriocin : Nisin F

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GENE SEQUENCEE
PubMed=18039827;DOI=10.1128/AEM.01862-07
de Kwaadsteniet M, Ten Doeschate K, Dicks L M T.
"Characterization of the Structural Gene Encoding Nisin F, a New Lantibiotic Produced by a Lactococcus lactis subsp. lactis Isolate from Freshwater Catfish (Clarias gariepinus)", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74(2):547-549.

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Sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 ITSISLCTPG CKTGALMGCN MKTATCNCSV HVSK
Wheel representation
Composition
Formula C140 H243 N41 O46 S7
Absent amino acids DEFQRWY
Common amino acids CT
Mass (Da) 3479.71
Net charge +4
Isoelectric point 8.51
Basic residues 4
Acidic residues 0
Hydrophobic residues 8
Polar residues 19
Aliphatic residues 6
Tiny residues 9
Boman Index -15.74
Hydropathy Index 0.4
Aliphatic Index 68.82
Instability Index 13.45 (stable)
Half Life Mammalian : 20 hour
Yeast : 30 min
E. coli : >10 hour
Extinction Coefficient 0 M-1 cm-1
Absorbance 280nm 7.58
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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