View bacteriocin : Subpeptin JM4-B Bookmark and Share

Accession BAC006
Name Subpeptin JM4-B
Gene Unidentified
Class Unclassified
Producer Organism Bacillus subtilis [Gram-positive]
Taxonomy BacteriaFirmicutesBacillalesBacillaceaeBacillus
Target organisms Gram-positive & Gram-negative bacteria: Salmonella - Bacillus cereus - Bacillus megaterium - Lactobacillus casei - Lactobacillus viridescens - Micrococcus flavus - Corynebacterium glutamicum - Cosmarium crenatum - Leuconostoc mesenteroides - Enterococcus faecalis - Shigella flexneri - Staphylococus aureus - Bacillus thuringiensis
Swiss-Prot Entry P83879
PDB Entry Unknown
Description Mode of action:
maximum activity is reached at the stationary phase. NOTE=Ref.1.
[Ref. 1]
PROTEIN SEQUENCE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MASS SPECTROMETRY, STRAIN=JM4

Wu S.-M., Sun D.-D., Jia S.-F., Chen M.-L., Huan L.-D.
"Purification and characterization of two novel antimicrobial peptides.", Submitted (MAR-2004) to Swiss-Prot.

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Sequence
........10........20
| |
XXKEIXHIFHDN
Wheel representation
Composition
AA N° %

AA N° %

AA N° %
Ala 0 .00%

Ile 2 16.67%

Arg 0 .00%
Cys 0 .00%

Lys 1 8.33%

Ser 0 .00%
Asp 1 8.33%

Leu 0 .00%

Thr 0 .00%
Glu 1 8.33%

Met 0 .00%

Val 0 .00%
Phe 1 8.33%

Asn 1 8.33%

Trp 0 .00%
Gly 0 .00%

Pro 0 .00%

Tyr 0 .00%
His 2 16.67%

Gln 0 .00%

Xaa 3 25.00%
Total 12
Formula C52 H71 N15 O12
Absent amino acids ACGLMPQRSTVWY
Common amino acids X
Mass (Da) 1446.42
Net charge +1
Isoelectric point 6.5
Basic residues 3
Acidic residues 2
Hydrophobic residues 3
Polar residues 1
Aliphatic residues 2
Tiny residues 0
Boman Index -24.22
Hydropathy Index -0.75
Aliphatic Index 65
Instability Index 58.5 (unstable)
Extinction Coefficient 0 M-1 cm-1
Absorbance 280nm 0
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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