BACTIBASE Database : PlnJ details

View bacteriocin : PlnJ

General data
References
Structural data
Physicochemical data
Comments

Accession	BAC027
Name	PlnJ
Gene	plnJ; OrderedLocusNames=lp_0406
Class	Class IIb
Producer Organism	Lactobacillus plantarum [Gram-positive]
Taxonomy	BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organisms	~~Unavailable data~~
Swiss-Prot Entry	P71461 Q7AKW6
PDB Entry	2KHF resolved by NMR. Structure in dpc micelles 2KHG resolved by NMR. (TFE)
Other databases	*EMBL* X94434 *EMBL* AL935253 *EMBL* AF522077 *GenomeReviews* AL935263_GR *BioCyc* LPLA220668:LP_0406-MONOMER

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=C11
MEDLINE=94161498;PubMed=8117074;
Diep B.D., Havarstein L.S., Nissen-Meyer J., Nes F.I.
"The gene encoding plantaricin A, a bacteriocin from Lactobacillus plantarum C11, is located on the same transcription unit as an agr- like regulatory system.", Appl. Environ. Microbiol. 60:160-166(1994).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=C11
MEDLINE=96345611;PubMed=8755874;
Diep D.B., Havarstein L.S., Nes I.F.
"Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11.", J. Bacteriol. 178:4472-4483(1996).

[Ref. 3] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], STRAIN=NCIMB 8826 / WCFS1
MEDLINE=22480296;PubMed=12566566;DOI=10.1073/pnas.0337704100
Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing
"Complete genome sequence of Lactobacillus plantarum WCFS1", Proc. Natl. Acad. Sci. U.S.A. 100 (4):1990-5 (2003).

[Ref. 4] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=C11
MEDLINE=96414465;PubMed=8817486;
Diep D.B., Haavarstein S.L., Nes I.F.
"A bacteriocin-like peptide induces bacteriocin synthesis in Lactobacillus plantarum C11.", Mol. Microbiol. 18:631-639(1995).

[Ref. 5] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=NC8
PubMed=14973042;DOI=10.1128/JB.186.5.1556-1564.2004
Maldonado A., Jimenez-Diaz R., Ruiz-Barba J.L.
"Induction of plantaricin production in Lactobacillus plantarum NC8 after coculture with specific gram-positive bacteria is mediated by an autoinduction mechanism.", J. Bacteriol. 186:1556-1564(2004).

Sequence

........10	........20	........30
\|	\|	\|
GAWKNFWSSL	RKGFYDGEAG	RAIRR

Wheel representation

Structure

Composition

AA	N°	%	AA	N°	%	AA	N°	%
Ala	3	12.00%	Ile	1	4.00%	Arg	4	16.00%
Cys	0	.00%	Lys	2	8.00%	Ser	2	8.00%
Asp	1	4.00%	Leu	1	4.00%	Thr	0	.00%
Glu	1	4.00%	Met	0	.00%	Val	0	.00%
Phe	2	8.00%	Asn	1	4.00%	Trp	2	8.00%
Gly	4	16.00%	Pro	0	.00%	Tyr	1	4.00%
His	0	.00%	Gln	0	.00%
Total	25

Formula	C₁₃₃ H₁₉₈ N₄₂ O₃₄
Absent amino acids	CHMPQTV
Common amino acids	GR
Mass (Da)	2947.69

Net charge	+4
Isoelectric point	11.51
Basic residues	6
Acidic residues	2
Hydrophobic residues	9
Polar residues	8
Aliphatic residues	2
Tiny residues	9

Boman Index	-70.24
Hydropathy Index	-0.93
Aliphatic Index	43.2
Instability Index	54.61 (unstable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	12490 M^-1 cm^-1
Absorbance 280nm	520.42

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).

Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).

Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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