BACTIBASE Database : curvacin-A details

View bacteriocin : curvacin-A

General data
References
Structural data
Physicochemical data
Comments

Accession	BAC065
Name	curvacin-A
Gene	curA
Class	class IIA/YGNGV
Producer Organism	Lactobacillus curvatus [Gram-positive]
Taxonomy	BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organisms	closely related Lactobacilli - Listeria monocytogenes - Listeria innocua - Listeria ivanovii - Enterococcus faecalis - Carnobacterium sp - Brocothrix thermosphacta
Swiss-Prot Entry	P0A311
PDB Entry	2A2B resolved by NMR.
Other databases	*EMBL* S67323 *EMBL* X72223 *InterPro* IPR002633 *InterPro* IPR010133 *Pfam* PF01721 *ProDom* PD004452 *TIGRFAMs* TIGR01847

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LTH1174
MEDLINE=94058500;PubMed=7694558;DOI=10.1007/BF00292077
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174.", Arch. Microbiol. 160:279-283(1993).

[Ref. 2]

PRELIMINARY PARTIAL PROTEIN SEQUENCE OF 19-59, STRAIN=LTH1174

Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).

[Ref. 3] | View abstract | Export citation

Mutational Analysis / Structure-function relationships
PubMed=18791005;DOI=10.1128/AEM.01068-08
Haugen HS, Kristiansen PE, Fimland G, Nissen-Meyer J
"Mutational Analysis of the Class IIa Bacteriocin Curvacin A and Its Orientation in Target Cell Membranes.", Appl. Environ. Microbiol. 74(21):6766-73 (2008).

Sequence

........10	........20	........30	........40	........50
\|	\|	\|	\|	\|
ARSYGNGVYC	NNKKCWVNRG	EATQSIIGGM	ISGWASGLAG	M

Wheel representation

Structure

Composition

AA	N°	%	AA	N°	%	AA	N°	%
Ala	4	9.76%	Ile	3	7.32%	Arg	2	4.88%
Cys	2	4.88%	Lys	2	4.88%	Ser	4	9.76%
Asp	0	.00%	Leu	1	2.44%	Thr	1	2.44%
Glu	1	2.44%	Met	2	4.88%	Val	2	4.88%
Phe	0	.00%	Asn	4	9.76%	Trp	2	4.88%
Gly	8	19.51%	Pro	0	.00%	Tyr	2	4.88%
His	0	.00%	Gln	1	2.44%
Total	41

Formula	C₁₈₄ H₂₈₈ N₅₆ O₅₆ S₄
Absent amino acids	DFHP
Common amino acids	G
Mass (Da)	4327.58

Net charge	+3
Isoelectric point	9.37
Basic residues	4
Acidic residues	1
Hydrophobic residues	12
Polar residues	21
Aliphatic residues	6
Tiny residues	16

Boman Index	-41.86
Hydropathy Index	-0.19
Aliphatic Index	61.95
Instability Index	27.63 (stable)
Half Life	Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	14105 M^-1 cm^-1
Absorbance 280nm	352.63

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).

Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).

Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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