BACTIBASE Database : Divergicin M35 details

View bacteriocin : Divergicin M35

General data
References
Structural data
Physicochemical data
Comments

Accession	BAC070
Name	Divergicin M35
Related Entries	Divergicin 750 Divergicin A
Gene	~~Unidentified~~
Class	class IIa/YGNGV
Producer Organism	Carnobacterium divergens (Lactobacillus divergens) [Gram-positive]
Taxonomy	BacteriaFirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium
Target organisms	Listeria monocytogenes - Listeria seeligeri - Listeria welshimeri - Listeria grayi - Listeria murrayi - Listeria ivanovii - Listeria innocua - Carnobacterium divergens - Carnobacterium piscicola - No antibacterial activity against Lactobacillus lactis - Pediococcus acidilactici - Pediococcus pentosaceus - Lactobacillus salivarius - Lactobacillus delbruekii - Lactobacillus acidophilus - Lactobacillus plantarum - Lactobacillus casei - Lactobacillus rhamnosus - Streptomyces thermophilus - Propionibacterium freudenreichii - Bifidobacterium breve - Escherichia coli
Swiss-Prot Entry	P84962
PDB Entry	~~Unknown~~
Other databases	GO GO:0005576 GO GO:0050830 *InterPro* IPR002633 *Pfam* PF01721 *ProDom* PD004452 *PROSITE* PS60030
Description	Has potential for use as a biopreservative for inhibiting Listeria monocytogenes in seafood products.

[Ref. 1] | View abstract | Export citation

PROTEIN SEQUENCE, STRAIN=M35
PubMed=15541799;
Tahiri I, Desbiens M, Benech R, Kheadr E, Lacroix C, Thibault S, Ouellet D, Fliss I.
"Purification, characterization and amino acid sequencing of divergicin M35: a novel class IIa bacteriocin produced by Carnobacterium divergens M35.", Int J Food Microbiol. 97(2):123-36(2004).

[Ref. 2] | View abstract | Export citation

MODE OF ACTION, STRAIN=M35
PubMed=16405682;
Naghmouchi K, Drider D, Kheadr E, Lacroix C, Prevost H, Fliss I.
"Multiple characterizations of Listeria monocytogenes sensitive and insensitive variants to divergicin M35, a new pediocin-like bacteriocin.", J Appl Microbiol. 2006;100(1):29-39.

[Ref. 3] | View abstract | Export citation

MODE OF ACTION, STRAIN=M35
PubMed=17578415;DOI=10.1111/j.1365-2672.2006.03206.x
Naghmouchi K., Drider D., Fliss I.
"Action of divergicin M35, a class IIa bacteriocin, on liposomes and Listeria.", Journal of Applied Microbiology 102(6):1508-17

Sequence

........10	........20	........30	........40	........50
\|	\|	\|	\|	\|
TKYYGNGVYC	NSKKCWVDWG	TAQGCIDVVI	GQLGGGIPGK	GKC

Wheel representation

Composition

AA	N°	%	AA	N°	%	AA	N°	%
Ala	1	2.33%	Ile	3	6.98%	Arg	0	.00%
Cys	4	9.30%	Lys	5	11.63%	Ser	1	2.33%
Asp	2	4.65%	Leu	1	2.33%	Thr	2	4.65%
Glu	0	.00%	Met	0	.00%	Val	4	9.30%
Phe	0	.00%	Asn	2	4.65%	Trp	2	4.65%
Gly	10	23.26%	Pro	1	2.33%	Tyr	3	6.98%
His	0	.00%	Gln	2	4.65%
Total	43

Formula	C₂₀₀ H₃₀₈ N₅₄ O₅₈ S₄
Absent amino acids	EFHMR
Common amino acids	G
Mass (Da)	4543.96

Net charge	+3
Isoelectric point	8.65
Basic residues	5
Acidic residues	2
Hydrophobic residues	11
Polar residues	22
Aliphatic residues	8
Tiny residues	12

Boman Index	-21.68
Hydropathy Index	-0.19
Aliphatic Index	65.58
Instability Index	-3.74 (stable)
Half Life	Mammalian : 7.2 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	15720 M^-1 cm^-1
Absorbance 280nm	374.29

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).

Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).

Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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