BACTIBASE Database : Enterocin I (Enterocin L50A) details

View bacteriocin : Enterocin I (Enterocin L50A)

General data
References
Structural data
Physicochemical data
Comments

Accession	BAC080
Name	Enterocin I (Enterocin L50A)
Related Entries	Enterocin Q Enterocin P Enterocin A Enterocin B Enterocin 1071A Enterocin CRL35 Enterocin SE-K4 Enterocin AS-48 (BACTERIOCIN AS-48)Enterocin 96 Enterocin E-760 Enterocin-HF
Gene	entI; Synonyms=entL50A
Class	bacteriocins without sequence leader
Producer Organism	Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy	BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organisms	clostridia - propionibacteria - Listeria monocytogenes - Lactobacillus sakei - Enterococcus faecium - Pediococcus acidilactici - Pediococcus pentosaceus
Swiss-Prot Entry	O53031
PDB Entry	~~Unknown~~
Other databases	*EMBL* Y16413 *EMBL* AJ223633

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=6T1a
MEDLINE=99054929;PubMed=9835578;
Floriano B., Ruiz-Barba J.L., Jimenez-Diaz R.
"Purification and genetic characterization of enterocin I from Enterococcus faecium 6T1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins.", Appl. Environ. Microbiol. 64:4883-4890(1998).

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=L50
MEDLINE=98215162;PubMed=9555877;
Cintas L.M., Casaus P., Holo H., Hernandez P.E., Nes I.F., Havarstein L.S.
"Enterocins L50A and L50B, two novel bacteriocins from Enterococcus faecium L50, are related to staphylococcal hemolysins.", J. Bacteriol. 180:1988-1994(1998).

Sequence

........10	........20	........30	........40	........50
\|	\|	\|	\|	\|
MGAIAKLVAK	FGWPIVKKYY	KQIMQFIGEG	WAINKIIEWI	KKHI

Wheel representation

Composition

AA	N°	%	AA	N°	%	AA	N°	%
Ala	4	9.09%	Ile	9	20.45%	Arg	0	.00%
Cys	0	.00%	Lys	8	18.18%	Ser	0	.00%
Asp	0	.00%	Leu	1	2.27%	Thr	0	.00%
Glu	2	4.55%	Met	2	4.55%	Val	2	4.55%
Phe	2	4.55%	Asn	1	2.27%	Trp	3	6.82%
Gly	4	9.09%	Pro	1	2.27%	Tyr	2	4.55%
His	1	2.27%	Gln	2	4.55%
Total	44

Formula	C₂₅₂ H₃₉₂ N₆₀ O₅₄ S₂
Absent amino acids	CDRST
Common amino acids	I
Mass (Da)	5209.14

Net charge	+7
Isoelectric point	10.68
Basic residues	9
Acidic residues	2
Hydrophobic residues	21
Polar residues	7
Aliphatic residues	12
Tiny residues	8

Boman Index	5.25
Hydropathy Index	0.2
Aliphatic Index	110.91
Instability Index	26.65 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	19480 M^-1 cm^-1
Absorbance 280nm	453.02

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).

Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).

Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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