BACTIBASE Database : Enterocin A details

View bacteriocin : Enterocin A

General data
References
Structural data
Physicochemical data
Comments

Accession	BAC088
Name	Enterocin A
Related Entries	Enterocin Q Enterocin P Enterocin I (Enterocin L50A)Enterocin B Enterocin 1071A Enterocin CRL35 Enterocin SE-K4 Enterocin AS-48 (BACTERIOCIN AS-48)Enterocin 96 Enterocin E-760 Enterocin-HF
Gene	entA
Class	Class IIa, IIc (problematic)
Producer Organism	Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy	BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organisms	Enterococcus - Lactobacillus - Pediococcus - Listeria monocytogenes
Swiss-Prot Entry	Q47784
PDB Entry	~~Unknown~~
Other databases	*EMBL* X94181 *EMBL* AB038464 *EMBL* AF099088 *HSSP* P34034 GO GO:0005576 GO GO:0042742 *InterPro* IPR002633 *Pfam* PF01721 *ProDom* PD004452

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=CTC492
MEDLINE=96209231;PubMed=8633865;
Aymerich T., Holo H., Havarstein L.S., Hugas M., Garriga M., Nes I.F.
"Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins.", Appl. Environ. Microbiol. 62:1676-1682(1996).

[Ref. 2]

NUCLEOTIDE SEQUENCE, STRAIN=CTC492

Harvarstein L.
"", Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.

[Ref. 3]

NUCLEOTIDE SEQUENCE, STRAIN=N15

Uchiyama K., Losteinkit C., Shioya S.
"Characterization of bacteriocin produced by Enterococcus faecium N15 and cloning of the related genes.", Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.

[Ref. 4] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=DPC1146
MEDLINE=99203104;PubMed=10103244;
O'Keeffe T., Hill C., Ross R.P.
"Characterization and heterologous expression of the genes encoding enterocin A production, immunity, and regulation in Enterococcus faecium DPC1146.", Appl. Environ. Microbiol. 65:1506-1515(1999).

Sequence

........10	........20	........30	........40	........50
\|	\|	\|	\|	\|
TTHSGKYYGN	GVYCTKNKCT	VDWAKATTCI	AGMSIGGFLG	GAIPGKC

Wheel representation

Composition

AA	N°	%	AA	N°	%	AA	N°	%
Ala	4	8.51%	Ile	3	6.38%	Arg	0	.00%
Cys	4	8.51%	Lys	5	10.64%	Ser	2	4.26%
Asp	1	2.13%	Leu	1	2.13%	Thr	6	12.77%
Glu	0	.00%	Met	1	2.13%	Val	2	4.26%
Phe	1	2.13%	Asn	2	4.26%	Trp	1	2.13%
Gly	9	19.15%	Pro	1	2.13%	Tyr	3	6.38%
His	1	2.13%	Gln	0	.00%
Total	47

Formula	C₂₁₁ H₃₂₉ N₅₇ O₆₃ S₅
Absent amino acids	EQR
Common amino acids	G
Mass (Da)	4851.38

Net charge	+5
Isoelectric point	8.98
Basic residues	6
Acidic residues	1
Hydrophobic residues	12
Polar residues	26
Aliphatic residues	6
Tiny residues	15

Boman Index	-20.81
Hydropathy Index	-0.03
Aliphatic Index	54.04
Instability Index	25.08 (stable)
Half Life	Mammalian : 7.2 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	10220 M^-1 cm^-1
Absorbance 280nm	222.17

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).

Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).

Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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