BACTIBASE Database : subtilosin details

View bacteriocin : subtilosin

General data
References
Structural data
Physicochemical data
Comments

Accession	BAC098
Name	subtilosin
Related Entries	Subtilosin-A
Gene	sboX; OrderedLocusNames=BSU37360
Class	~~Unclassified~~
Producer Organism	Bacillus subtilis [Gram-positive]
Taxonomy	BacteriaFirmicutesBacillalesBacillaceaeBacillus
Target organisms	Bacillus subtilis - Enterococcus faecalis - Escherichia coli - Fusobacterium nucleatum - Listeria monocytogenes - Porphyromonas gingivalis - Pseudomonas aeruginosa - Staphylococcus aureus - Klebsiella pneumoniae - Bacillus cereus - Staphylococcus epidermidis - Kocuria rhizophila - Enterobacter aerogenes - Proteus mirabilis - Salmonella enterica Typhi - Streptococcus pyogenes - Streptococcus gordonii Challis - Shigella sonnei
Swiss-Prot Entry	Q7WY57
PDB Entry	~~Unknown~~
Other databases	*EMBL* Z99123 *GenomeReviews* AL009126_GR *SubtiList* BG14182

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], STRAIN=168
MEDLINE=98044033;PubMed=9384377;DOI=10.1038/36786
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter
"?",

[Ref. 2] | View abstract | Export citation

IDENTIFICATION, AND INDUCTION, STRAIN=168 / JH642
MEDLINE=20270159;PubMed=10809709;DOI=10.1128/JB.182.11.3266-3273.2000
Zheng G., Hehn R., Zuber P.
"Mutational analysis of the sbo-alb locus of Bacillus subtilis: identification of genes required for subtilosin production and immunity.", J. Bacteriol. 182:3266-3273(2000).

[Ref. 3] | View abstract | Export citation

FUNCTION
PubMed=17213266;
Shelburne CE, An FY, Dholpe V, Ramamoorthy A, Lopatin DE, Lantz MS.
"The spectrum of antimicrobial activity of the bacteriocin subtilosin A.", J Antimicrob Chemother. 2007 Feb;59(2):297-300.

Sequence

........10	........20	........30	........40	........50	........60
\|	\|	\|	\|	\|	\|
MKLPVQQVYS	VYGGKDLPKG	HSHSTMPFLS	KLQFLTKIYL	LDIHTQPFFI

Wheel representation

Composition

AA	N°	%	AA	N°	%	AA	N°	%
Ala	0	.00%	Ile	3	6.00%	Arg	0	.00%
Cys	0	.00%	Lys	5	10.00%	Ser	4	8.00%
Asp	2	4.00%	Leu	7	14.00%	Thr	3	6.00%
Glu	0	.00%	Met	2	4.00%	Val	3	6.00%
Phe	4	8.00%	Asn	0	.00%	Trp	0	.00%
Gly	3	6.00%	Pro	4	8.00%	Tyr	3	6.00%
His	3	6.00%	Gln	4	8.00%
Total	50

Formula	C₂₇₄ H₄₂₁ N₆₅ O₆₉ S₂
Absent amino acids	ACENRW
Common amino acids	L
Mass (Da)	5812.7

Net charge	+6
Isoelectric point	10.01
Basic residues	8
Acidic residues	2
Hydrophobic residues	17
Polar residues	13
Aliphatic residues	13
Tiny residues	7

Boman Index	-22.3
Hydropathy Index	0.02
Aliphatic Index	95.4
Instability Index	37.77 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	4470 M^-1 cm^-1
Absorbance 280nm	91.22

Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).

Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).

Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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