View bacteriocin : Enterocin CRL35 Bookmark and Share

[Ref. 1] | View abstract | Export citation
PROTEIN SEQUENCE
PubMed=8695065;
Far?as ME, Far?as RN, de Ruiz Holgado AP, Sesma F.
"Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35. Lett Appl Microbiol. 1996, 22(6):417-9.",

[Ref. 2] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=CRL35
PubMed=15215149;DOI=10.1128/AAC.48.7.2778-2781.2004
Saavedra L., Minahk C., De Ruiz Holgado A.P., Sesma F.
"Enhancement of the Enterocin CRL35 Activity by a Synthetic Peptide Derived from the NH2-Terminal Sequence.", Antimicrob. Agents Chemother. 48:2778-2781(2004).

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Sequence
........10........20........30........40........50
| | | | |
KYYGNGVSCNKKGCSVDWGKAIGIIGNNSAANLATGGAAGWKS
Wheel representation
Composition
AA N° %

AA N° %

AA N° %
Ala 6 13.95%

Ile 3 6.98%

Arg 0 .00%
Cys 2 4.65%

Lys 5 11.63%

Ser 4 9.30%
Asp 1 2.33%

Leu 1 2.33%

Thr 1 2.33%
Glu 0 .00%

Met 0 .00%

Val 2 4.65%
Phe 0 .00%

Asn 5 11.63%

Trp 2 4.65%
Gly 9 20.93%

Pro 0 .00%

Tyr 2 4.65%
His 0 .00%

Gln 0 .00%
Total 43
Formula C186 H291 N55 O58 S2
Absent amino acids EFHMPQR
Common amino acids G
Mass (Da) 4308.55
Net charge +4
Isoelectric point 9.81
Basic residues 5
Acidic residues 1
Hydrophobic residues 14
Polar residues 23
Aliphatic residues 6
Tiny residues 19
Boman Index -31.82
Hydropathy Index -0.253
Aliphatic Index 15.04
Instability Index 6.27 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 14105 M-1 cm-1
Absorbance 280nm 335.83
Red solid plot : values according to the hydrophobicity scale of Kyte and Doolittle (reference paper).


Yellow dashed plot : Experimentally determined hydrophobicity scale for proteins at membrane interfaces(reference paper).


Green dotted-dashed plot : prediction of transmembrane helices (reference paper). In this scale (unlike the others), more negative values reflect greater hydrophobicity.

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